[Research Article] Replication of human noroviruses in stem cell –derived human enteroids

We report the successful cultivation of multiple HuNoV strains in enterocytes in stem cell–derived, nontransformed human intestinal enteroid monolayer cultures. Bile, a critical factor of the intestinal milieu, is required for strain-dependent HuNoV replication. Lack of appropriate histoblood group antigen expression in intestinal cells restricts virus replication, and infectivity is abrogated by inactivation (e.g., irradiation, heating) and serum neutralization. This culture system recapitulates the human intestinal epithelium, permits human host-pathogen studies of previously noncultivatable pathogens, and allows the assessment of methods to prevent and treat HuNoV infections. Authors: Khalil Ettayebi, Sue E. Crawford, Kosuke Murakami, James R. Broughman, Umesh Karandikar, Victoria R. Tenge, Frederick H. Neill, Sarah E. Blutt, Xi-Lei Zeng, Lin Qu, Baijun Kou, Antone R. Opekun, Douglas Burrin, David Y. Graham, Sasirekha Ramani, Robert L. Atmar, Mary K. Estes
Source: ScienceNOW - Category: Science Authors: Source Type: news