Cordycepin attenuates traumatic brain injury-induced impairments of blood-brain barrier integrity in rats.

Cordycepin attenuates traumatic brain injury-induced impairments of blood-brain barrier integrity in rats.
Brain Res Bull. 2016 Sep 16;
Authors: Yuan J, Wang A, He Y, Si Z, Xu S, Zhang S, Wang K, Wang D, Liu Y
Abstract
Loss of blood-brain barrier (BBB) integrity is a downstream event caused by traumatic brain injury (TBI). BBB integrity is affected by certain physiological conditions, including inflammation and oxidative stress. Cordycepin is a susbtance with anti-inflammatory and anti-oxidative effects. Therefore, it is necessary to investigate whether cordycepin affects TBI-induced impairments of BBB integrity. Using TBI rats as the in vivo model and applying multiple techniques, including stroke severity evaluation, Evans blue assessment, quantitative real-time PCR, Western blotting and ELISA, we investigated the dose-dependent protective effects of cordycepin on the TBI-induced impairments of BBB integrity. Cordycepin treatment attenuated the TBI-induced impairments in a dose-dependent manner, and played a role in protecting BBB integrity. Cordycepin was able to alleviate TBI-induced loss of tight junction proteins zonula occludens protein-1 (ZO-1) and occludin, which are important for BBB integrity. Moreover, cordycepin suppressed pro-inflammatory factors, including IL-1β, iNOS, MPO and MMP-9, and promoted anti-inflammation-associated factors arginase 1 and IL-10. Furthermore, cordycepin inhibited NADPH oxidase (NOX) expression...

Source: Brain Research Bulletin - September 15, 2016 Category: Neurology Authors: Yuan J, Wang A, He Y, Si Z, Xu S, Zhang S, Wang K, Wang D, Liu Y Tags: Brain Res Bull Source Type: research