Molecular typing and genetic characterization of Mycobacterium avium subspecies hominissuis isolates from humans and swine in Japan.

Molecular typing and genetic characterization of Mycobacterium avium subspecies hominissuis isolates from humans and swine in Japan. J Med Microbiol. 2016 Sep 6; Authors: Adachi T, Ichikawa K, Inagaki T, Moriyama M, Nakagawa T, Ogawa K, Hasegawa Y, Yagi T Abstract Mycobacterium avium subspecies hominissuis (MAH) causes disease in both humans and swine; however, the genetic variations in MAH isolates are unclear. The aim of this study was to elucidate the genetic variations in MAH isolates from humans and swine in Japan. We analyzed the 16S-23S rDNA internal transcribed spacer (ITS) sequence and variable number of tandem repeats (VNTR) using the Mycobacterium avium tandem repeat (MATR) loci, prevalence of ISMav6 and clarithromycin (CLA) resistance for MAH isolates from patients with pulmonary MAC (pMAC) disease (n = 69), HIV-seropositive and blood culture-positive (HIV-MAC) patients (n = 28), and swine (n = 23). In the minimum spanning tree (MST) based on VNTR analysis, swine MAC isolates belonged to a cluster distinguishable from that of human pMAC isolates. Isolates from HIV-MAC were scattered throughout both clusters. The major three distinct sequevars, Mav-A, B, F were determined according to 16S-23S rDNA ITS sequence analysis in addition to three new sequevars, Mav-Q, R, and S. Mav-A and Mav-F comprised the majority of human pMAC strains, in contrast, Mav-B was predominated in swine isolates. Distribution of ITS sequevars in the ...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research