Analysis of Epstein-Barr virus and cellular gene expression during the early phases of EBV lytic induction.

Analysis of Epstein-Barr virus and cellular gene expression during the early phases of EBV lytic induction. J Med Microbiol. 2016 Sep 12; Authors: Auburn H, Zuckerman M, Smith M Abstract AbstractIn order to develop novel host/pathogen real- time PCR assays for routine diagnostic use, early gene expression patterns from both Epstein-Barr virus (EBV) and Raji cells were examined after inducing the lytic life cycle using 12-O-tetradecanoyl-13-phorbol ester (TPA) and sodium butyrate. Real-time PCR identified several highly induced (>90-fold) EBV lytic genes over a 48 hr time-course during the lytic induction phase. Latent genes were induced at low levels during this phase. The cellular response to lytic viral replication is poorly understood. Whole human genome microarray analysis identified 113 cellular genes regulated ≥2-fold by EBV, including 63 up- and 46 downregulated genes, over a 24 h time-course post-induction. The most upregulated gene was CHI3L1, a chitinase-3-like 1 protein (18.1-fold; P<0.0084) and the most downregulated gene was TYMS, a thymidylate synthetase (-7.6-fold). Gene Ontology Enrichment analysis using Metacore software revealed cell cycle (core), cell cycle (role of APC in cell cycle regulation) and lymphatic diseases as the most significantly represented biological network processes, canonical pathways and disease biomarkers, respectively. Chemotaxis, DNA damage and inflammation (IL-4 signalling) together ...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research