Reliable Next-Generation Sequencing of Formalin-Fixed, Paraffin-Embedded Tissue Using Single Molecule Tags

Sequencing of tumor DNA to detect genetic aberrations is getting increasingly important, not only to refine cancer diagnoses but also to predict response to targeted treatments. Next-generation sequencing is widely adopted in diagnostics for the analyses of DNA extracted from routinely processed formalin-fixed, paraffin-embedded tissue, fine-needle aspirates, or cytologic smears. PCR-based enrichment strategies are usually required to obtain sufficient read depth for reliable detection of genetic aberrations.

http://jmd.amjpathol.org/article/S1525-1578(16)30141-6/fulltext?rss=yes

Source: Journal of Molecular Diagnostics - September 12, 2016 Category: Pathology Authors: Astrid Eijkelenboom, Eveline Kamping, Annemiek Kastner-van Raaij, Sandra Hendriks-Cornelissen, Kornelia Neveling, Roland Kuiper, Alexander Hoischen, Marcel Nelen, Marjolijn Ligtenberg, Bastiaan Tops Tags: Regular Article Source Type: research