Molecular detection of Sarcocystis aucheniae in the blood of llamas from Argentina.

Molecular detection of Sarcocystis aucheniae in the blood of llamas from Argentina. Rev Argent Microbiol. 2016 Sep 8; Authors: Martin M, Decker Franco C, Romero S, Carletti T, Schnittger L, Florin-Christensen M Abstract Sarcocystis aucheniae are apicomplexan protozoa that infect South American camelids (SACs), giving rise to macroscopic cysts similar to rice grains in skeletal muscles. Visual detection of macrocysts in slaughtered animals hampers commercialization of SAC meat, a highly relevant economic exploitation for Andean rural families. Importantly, the consumption of undercooked S. aucheniae-infested meat causes gastroenteritis. A carnivore definitive host, possibly the dog, acquires the parasite when feeding on infected SAC meat, and later eliminates infective oocysts in its feces. The parasite cycle is completed when SACs ingest contaminated water or pastures. We hypothesized that parasite DNA can be detected in SAC blood using molecular methods. In order to test this hypothesis, a seminested PCR format was specifically designed to target the hypervariable 18S rRNA gene region of S. aucheniae. PCR conditions were optimized using genomic DNA extracted from macrocyst bradyzoites. A detection limit of up to 1 parasite in 10μl of llama blood was established based on DNA samples extracted from aliquots of S. aucheniae bradyzoite-spiked non-infected llama blood. The seminested PCR allowed to detect natural infections of S. aucheniae in llama blood s...
Source: Revista Argentina de Microbiologia - Category: Microbiology Tags: Rev Argent Microbiol Source Type: research

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Source: Parasites and Vectors - Category: Microbiology Authors: Tags: Research Source Type: research
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Source: Scandinavian Journal of Gastroenterology - Category: Gastroenterology Tags: Scand J Gastroenterol Source Type: research
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Source: IPS Inter Press Service - Health - Category: International Medicine & Public Health Authors: Tags: Civil Society Development & Aid Editors' Choice Environment Featured Headlines Health Integration and Development Brazilian-style Latin America & the Caribbean Population Poverty & SDGs Projects Regional Categories TerraViva Unit Source Type: news
The objective of this study was to evaluate the management and health care utilization of patients following GI panel compared with conventional stool testing. We performed a retrospective comparative analysis of 9,402 patients who underwent testing with the FilmArray GI panel from March 2015 through May 2017 and 5,986 patients who underwent conventional stool testing from December 2012 through February 2015. GI panel was positive in 2,746 exams (29.2%) compared with 246 exams (4.1%) with conventional testing. Within 30 days following stool testing, compared with patients who received a conventional stool test, patients wh...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Bacteriology Source Type: research
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Source: Archives of Pathology and Laboratory Medicine - Category: Laboratory Medicine Authors: Tags: Arch Pathol Lab Med Source Type: research
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Source: The Journal of Parasitology - Category: Parasitology Authors: Tags: J Parasitol Source Type: research
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Source: Blood - Category: Hematology Authors: Tags: 203. Lymphocytes, Lymphocyte Activation, and Immunodeficiency, including HIV and Other Infections Source Type: research
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Source: PLOS Currents Disasters - Category: International Medicine & Public Health Authors: Source Type: research
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Source: Journal of Ethnopharmacology - Category: Drugs & Pharmacology Source Type: research
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