Quantitation of IgG kappa and IgG lambda in the cerebrospinal fluid by sandwich ELISA method.

Quantitation of IgG kappa and IgG lambda in the cerebrospinal fluid by sandwich ELISA method. J Immunoassay Immunochem. 2016 Sep 9; Authors: Zeman D, Kušnierová P, Bojková J, Všianský F, Zapletalová O Abstract IgG kappa and IgG lambda concentrations were quantified in 96 paired CSF and sera using Hevylite™ antibodies in an in-house developed sandwich ELISA method. In 56 of these samples, the results were compared with a qualitative isoelectric focusing/affinity-mediated immunoblotting assay for oligoclonal IgG kappa and IgG lambda. Normal IgG kappa/lambda ratio in the CSF was the same as in serum. In 19/33 patients with intrathecal oligoclonal IgG synthesis, skewed IgG kappa/lambda ratio was observed (increased in 16 and decreased in 3 cases). The analysis of light chain composition of intrathecally synthesised immunoglobulins could contribute to our understanding of intrathecal humoral immune response, although its diagnostic utility is limited. PMID: 27611231 [PubMed - as supplied by publisher]
Source: Journal of Immunoassay and Immunochemistry - Category: Biochemistry Tags: J Immunoassay Immunochem Source Type: research
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