Localization of rem2 in the central nervous system of the adult rainbow trout (Oncorhynchus mykiss)

Publication date: Available online 4 September 2016 Source:Journal of Chemical Neuroanatomy Author(s): Anna G. Downs, Katie R. Scholles, David M. Hollis Rem2 is member of the RGK (Rem, Rad, and Gem/Kir) subfamily of the Ras superfamily of GTP binding proteins known to influence Ca2+ entry into the cell. In addition, Rem2, which is found at high levels in the vertebrate brain, is also implicated in cell proliferation and synapse formation. Though the specific, regional localization of Rem2 in the adult mammalian central nervous system has been well-described, such information is lacking in other vertebrates. We sought to localize rem2 gene expression in the central nervous system. Because Rem2 is involved in neuronal processes where differences in the capacities between adults of the different vertebrate classes exist, we sought to localize the rem2 gene in the central nervous system of an adult anamniotic vertebrate, the rainbow trout (Oncorhynchus mykiss). In situ hybridization using a digoxigenin (DIG)-labeled RNA probe was used to identify the regional distribution of rem2 expression throughout the trout central nervous system, while real-time polymerase chain reaction (rtPCR) further supported these findings. Based on in situ hybridization, the regional distribution of rem2 occurred within each major subdivision of the brain and included large populations of rem2 expressing cells in the dorsal telencephalon of the cerebrum, the internal cellular layer of the olfactory bu...
Source: Journal of Chemical Neuroanatomy - Category: Neuroscience Source Type: research

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