Improving Accuracy of Urinary miRNA Quantification in Heparinized Patients Using Heparinase I Digestion

miRNAs have emerged as promising biomarkers because of their association with cell stress and diseases and their easy detection and stability in many body fluids. Because of the sensitivity, the method of choice to detect miRNAs is quantitative RT-PCR (RT-qPCR). Therapeutics, in particular circulating anticoagulants, are notorious for their inhibitory effect on RT-qPCR –based measurements. The effect of heparin contamination on inhibition of RT-qPCR from miRNAs isolated from urine has, however, never been investigated.

Source: Journal of Molecular Diagnostics - September 2, 2016 Category: Pathology Authors: Henk P. Roest, Cornelia J. Verhoeven, Jubi E. de Haan, Jeroen de Jonge, Jan N.M. IJzermans, Luc J.W. van der Laan Tags: Technical Advance Source Type: research