Shiga Toxin Subtypes and Virulence Genes Distributed among Escherichia coli Isolated from Cattle.

Shiga Toxin Subtypes and Virulence Genes Distributed among Escherichia coli Isolated from Cattle.
Jpn J Infect Dis. 2016 Aug 31;
Authors: Akiyama Y, Futai H, Saito E, Ogita K, Sakae H, Fukunaga M, Tsuji H, Chikahira M, Iguchi A
Abstract
Subtypes of stx1 and stx2 carried in 45 Shiga toxin-producing Escherichia coli (STEC) strains isolated from cattle were investigated using PCR. Only subtype stx1a was detected among all the stx1-positive strains. The major stx2 subtype was stx2a, and followed by stx2d, stx2c, stx2b and stx2g in decreasing order. stx2c was found in the strains of O157 and O174. stx2d was found in 11 strains; they were confirmed using DNA sequencing to carry both the activatable tail and the END motif, all of which were eae-negative and three contained stx2d as the only stx. stx2g was found in two strains in association with stx2a, estA1, and astA. In addition, seven hybrid strains of Shigatoxigenic and enterotoxigenic E. coli (STEC/ETEC) harboring both or one of stx1a and stx2a (stx1a/stx2a) and estA1 were detected. Among 27 serotypes of STEC strains isolated from cattle, O157:H7 and O109:H- strains were eae-positive. Other putative adhesin genes, such as saa, iha, espP, and lipAO113 were frequently detected in more than 12 serotypes.
PMID: 27580573 [PubMed - as supplied by publisher]

Source: Japanese Journal of Infectious Diseases - August 30, 2016 Category: Infectious Diseases Authors: Akiyama Y, Futai H, Saito E, Ogita K, Sakae H, Fukunaga M, Tsuji H, Chikahira M, Iguchi A Tags: Jpn J Infect Dis Source Type: research