Isolation and characterization of an Escherichia albertii producing three different toxins from a child with diarrhea.

Isolation and characterization of an Escherichia albertii producing three different toxins from a child with diarrhea. Jpn J Infect Dis. 2016 Aug 31; Authors: Hinenoya A, Yasuda N, Hibino T, Shima A, Nagita A, Tsukamoto T, Yamasaki S Abstract Here, we show that a bacterium isolated as a sole pathogen from a child with diarrhea harbor eae and 2 different cytolethal distending toxin genes (cdt) homologous to Escherichia coli cdt-I and cdt-II genes, respectively. The bacterium was originally identified as atypical E. coli by conventional biochemical tests. However, this bacterium was finally identified as E. albertii by multilocus sequence analysis, which is an only method being able to clearly differentiate E. coli from E. albertii. Shiga toxin 2f (stx2f) genes were also detected in the strain. Production of the 3 toxins was confirmed by cytotoxicity assay using eukaryotic cell lines and/or western blotting. This is the first report showing the biological activity of CDT-I, CDT-II, and Stx2f in E. albertii. PMID: 27580579 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research