Gonococcal Antimicrobial Susceptibility and Prevalence of blaTEM-1, blaTEM-135 Genes in Thailand.

Gonococcal Antimicrobial Susceptibility and Prevalence of blaTEM-1, blaTEM-135 Genes in Thailand. Jpn J Infect Dis. 2016 Aug 31; Authors: Tribuddharat C, Pongpech P, Charoenwatanachokchai A, Lokpichart S, Srifuengfung S, Sonprasert S Abstract We studied antimicrobial susceptibility and the current rates of blaTEM-1 and blaTEM-135. Neisseria gonorrhoeae 370 isolates were tested by using disk diffusion method and found 100% susceptible to cefixime, ceftriaxone, cefotaxime, ceftazidime, cefepime, spectinomycin and azithromycin. They were resistant to penicillin (85.7%), ciprofloxacin (88.0%), ofloxacin (97.4%), and tetracycline (89.1%). Penicillinase-producing N. gonorrhoeae (PPNG) was 83.8% which 70.0% was further identified as PPNG plus tetracycline resistant N. gonorrhoeae, with 13.8% being PPNG alone. Penicillin, tetracycline and ciprofloxacin were not recommended for treatment because these multidrug resistant gonococci were high prevalence (89.7%). Study of genes controlling enzyme beta-lactamase production (blaTEM-1 and blaTEM-135) was performed by using mismatch amplification mutation assay PCR method (MAMA-PCR) and DNA sequencing. The prevalence of beta-lactamase positive gonococci carried blaTEM-1 (69.6%) and blaTEM-135 (30.4%), indicated the significant increasing and spread of blaTEM-135 among gonococci in Thailand. PMID: 27580580 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research