Targeted Metabolomic Profiling Indicates Structure-based Perturbations in Serum Phospholipids in Children with Acetaminophen Overdose

Publication date: Available online 23 August 2016 Source:Toxicology Reports Author(s): Sudeepa Bhattacharyya, Lisa Pence, Ke Yan, Pritmohinder Gill, Chunqiao Luo, Lynda G. Letzig, Pippa M. Simpson, Gregory L. Kearns, Richard D. Beger, Laura P. James Phospholipids are an important class of lipids that act as building blocks of biological cell membranes and participate in a variety of vital cellular functions including cell signaling. Previous studies have reported alterations in phosphatidylcholine (PC) and lysophosphatidylcholine (lysoPC) metabolism in acetaminophen (APAP)-treated animals or cell cultures. However, little is known about phospholipid perturbations in humans with APAP toxicity. In the current study, targeted metabolomic analysis of 180 different metabolites including 14 lysoPCs and 73 PCs was performed in serum samples from children and adolescents hospitalized for APAP overdose. Metabolite profiles in the overdose group were compared to those of healthy controls and hospitalized children receiving low dose APAP for treatment of pain or fever (therapeutic group). PCs and lysoPCs with very long chain fatty acids (VLCFAs) were significantly decreased in the overdose group, while those with comparatively shorter chain lengths were increased in the overdose group compared to the therapeutic and control groups. All ether linked PCs were decreased in the overdose group compared to the controls. LysoPC-C26:1 was highly reduced in the overdose group and cou...
Source: Toxicology Reports - Category: Toxicology Source Type: research