Expression, purification and crystallization of a plant polyketide cyclase from Cannabis sativa

Plant polyketides are a structurally diverse family of natural products. In the biosynthesis of plant polyketides, the construction of the carbocyclic scaffold is a key step in diversifying the polyketide structure. Olivetolic acid cyclase (OAC) from Cannabis sativa L. is the only known plant polyketide cyclase that catalyzes the C2 – C7 intramolecular aldol cyclization of linear pentyl tetra- β -ketide-CoA to generate olivetolic acid in the biosynthesis of cannabinoids. The enzyme is also thought to belong to the dimeric α + β barrel (DABB) protein family. However, because of a lack of functional analysis of other plant DABB proteins and low sequence identity with the functionally distinct bacterial DABB proteins, the catalytic mechanism of OAC has remained unclear. To clarify the intimate catalytic mechanism of OAC, the enzyme was overexpressed in Escherichia coli and crystallized using the vapour-diffusion method. The crystals diffracted X-rays to 1.40   Å resolution and belonged to space group P3121 or P3221, with unit-cell parameters a = b = 47.3, c = 176.0   Å . Further crystallographic analysis will provide valuable insights into the structure – function relationship and catalytic mechanism of OAC.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: cannabinoids Cannabis sativa cyclase olivetolic acid research communications Source Type: research

Related Links:

(Institut national de la recherche scientifique - INRS) Fresh produce is a major vehicle for noroviruses, a group of viruses that are the most common cause of gastroenteritis in developed countries. However, the viruses are quite resistant to cold pasteurization treatments such as irradiation, which are used to destroy bacteria, moulds, parasites, and insects. The irradiation process uses gamma rays or X-rays to destroy these viruses but at the dose needed to eliminate them, it can affect the physicochemical properties of fresh produce.
Source: EurekAlert! - Biology - Category: Biology Source Type: news
Publication date: March 2020Source: Colloid and Interface Science Communications, Volume 35Author(s): Nida Khan, Deepak Kumar, Pramendra KumarAbstractGuar gum/Gelatin/Silver nanocomposite (GG/Gl/Ag-N-composite) was synthesized thoroughly in greenway via simple in situ method by maltose sugar reduction using the varying concentration of AgNO3 solution. The synthesized composite was characterized by Fourier Transform Infrared (FT-IR) spectroscopy, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and X-rays diffraction (XRD) analysis. The synthesized composites were tested for antibacterial activity ...
Source: Colloids and Interface Science Communications - Category: Nanotechnology Source Type: research
By SAURABH JHA, MD Slumdog TB No one knows who gave Rahul Roy tuberculosis. Roy’s charmed life as a successful trader involved traveling in his Mercedes C class between his apartment on the plush Nepean Sea Road in South Mumbai and offices in Bombay Stock Exchange. He cared little for Mumbai’s weather. He seldom rolled down his car windows – his ambient atmosphere, optimized for his comfort, rarely changed. Historically TB, or “consumption” as it was known, was a Bohemian malady; the chronic suffering produced a rhapsody which produced fine art. TB was fashionable in Victorian Britain...
Source: The Health Care Blog - Category: Consumer Health News Authors: Tags: Artificial Intelligence Health Tech Saurabh Jha TB tuberculosis Source Type: blogs
In conclusion, potential biological applications of PF-SeNPs were explored by antibacterial, antifungal, antioxidant and biocompatibility assays. Materials and Methods Chemicals and Reagents Sodium selenite (98%), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT), Tween 80, methanol, acetate buffer, tetracycline, nystatin, rhodamine 123, caspase-3 kit, Dulbecco’s modified Eagle media (DMEM), Folin-Ciocalteu reagent, 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS), Dulbecco’s phosphate buffered saline pH 7.4 (DPBS) and fetal bovine serum (FBS) w...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 25 April 2019Source: Journal of Molecular StructureAuthor(s): Arshad Farooq Butt, Muhammad Naeem Ahmed, Moazzam Hussain Bhatti, Muhammad Aziz Choudhary, Khurshid Ayub, Muhammad Nawaz Tahir, Tariq MahmoodAbstractHerein, we report the synthesis, characterization and biological evaluations of two novel organotin(IV) carboxylates (1 and 2). The structures of both complexes are confirmed by single crystal X-rays diffraction analysis. Density functional theory (DFT) studies are also executed to compare experimental (X-ray as well as spectroscopic) results with theoretical ones. Excellent correl...
Source: Journal of Molecular Structure - Category: Molecular Biology Source Type: research
In this study, we report low-cost, green synthesis of AgNPs using fresh fruit extract of Phyllanthus emblica. The biosynthesized AgNPs was confirmed and characterized by analysis of spectroscopy profile of the UV-visible and Energy dispersive spectrophotometer, Fourier transform infrared, X-ray diffraction pattern, and electron microscopy images examination. UV-visible spectra showed a surface resonance peak of 430 nm corresponding to the formation of AgNPs, and FTIR spectra confirmed the involvement of biological molecules in AgNPs synthesis. In spherical AgNPs, the particle size ranged from 19.8 to 92.8 nm and the averag...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Nancy Merino1,2,3, Heidi S. Aronson4, Diana P. Bojanova1, Jayme Feyhl-Buska1, Michael L. Wong5,6, Shu Zhang7 and Donato Giovannelli2,8,9,10* 1Department of Earth Sciences, University of Southern California, Los Angeles, CA, United States 2Earth-Life Science Institute, Tokyo Institute of Technology, Tokyo, Japan 3Biosciences and Biotechnology Division, Physical and Life Sciences Directorate, Lawrence Livermore National Lab, Livermore, CA, United States 4Department of Biology, University of Southern California, Los Angeles, CA, United States 5Department of Astronomy – Astrobiology Program, University of Wash...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
Publication date: Available online 13 February 2019Source: Journal of Photochemistry and Photobiology B: BiologyAuthor(s): Aziz Ur Rahman, Arif Ullah Khan, Qipeng Yuan, Yun Wei, Aftab Ahmad, Sadiq Ullah, Zia Ul Haq Khan, Saira Shams, Muhammad Tariq, Waqas AhmadAbstractMetal nanoparticles, synthesized using Phyto-constituents are the most economically and environmentally benign materials ever. Biogenic silver nanoparticles (AgNPs) from three fractions of Arisaema flavum tuber extract were synthesized and characterized by UV–visible spectroscopy, XRD (X-rays diffraction), FT-IR (Fourier transform infrared spectroscopy)...
Source: Journal of Photochemistry and Photobiology B: Biology - Category: Speech-Language Pathology Source Type: research
Although acute osteomyelitis is rare in neonates, it might result in severe sequelae such as joint destruction and growth failure if it is not diagnosed and treated early. However, few studies have focused on the clinical features and treatment of this disease. A retrospective review of 17 cases of neonatal osteomyelitis, for which the patients underwent medical treatment alone or combined with surgery at the Children's Hospital of Zhejiang University School of Medicine between January 2009 and September 2016, was conducted. Medical treatment included the use of antibiotics and supportive care. Surgery was performed in ca...
Source: Medicine - Category: Internal Medicine Tags: Research Article: Observational Study Source Type: research
Abstract The present study reports ecofriendly synthesis of CuO nanoparticles (NPs) using an extract of Rhus punjabensis as a reducing agent. NPs structural and composition analysis are evaluated by X-rays diffraction (XRD), Fourier transform infrared, Energy dispersive spectroscopy, Scanning electron microscopy, Transmission electron microscopy, and Thermal analysis. The NPs have pure single phase monoclinic geometry with spherical structure and high stability toward heat and with average particle size of about 36.6 and 31.27 nm calculated by XRD and SEM, respectively. NPs are tested for antibacterial, pro...
Source: Natural Product Research - Category: Biochemistry Authors: Tags: Nat Prod Res Source Type: research
More News: Biochemistry | Gastroenteritis | X-Ray