Interleukin 10 ( −1082 G/A) and (−819 C/T) gene polymorphisms in Egyptian women with polycystic ovary syndrome (PCOS)

Publication date: Available online 3 August 2016 Source:Meta Gene Author(s): Roba M. Talaat, Yasmin A. Mohamed, Ehab H. Mohamad, Marwa Elsharkawy, Adel A. Guirgis Cytokines play critical roles in the pathogenesis of Polycystic Ovarian Syndrome (PCOS). This work was designed to study the implication of IL10 gene polymorphisms (−1082 G/A and −819 C/T) on the susceptibility of Egyptian women to have PCOS. Rotterdam consensus criteria were used to diagnose PCOS patients. Genotyping was performed by single-stranded polymorphism-polymerase chain reaction (SSP-PCR) in 61 PCOS patients and 80 healthy controls, and IL-10 serum levels were measured using Enzyme linked immunosorbent assay (ELISA). The frequency of IL10 −1082 G/G (46%) genotype was significantly increased (p<0.001) while the frequency of −1082 A/A (16%) genotype was significantly decreased (p<0.05) in PCOS patients compared to controls (14% and 35% for G/G and A/A genotypes; respectively). G allele (65%) is significantly increased (p<0.01( in PCOS patients while A allele (61%) is significantly increased (p<0.001( in control subjects. The distribution of IL10 -819 T/T genotype was significantly increased (p<0.05) in PCOS group. G/G genotype (odd ratio (OR=5.322) with confidence interval (CI=2.364–11.982) and the G allele (OR=2.828 with CI=1.73–4.61) of −1082 G/A and T/T genotype of −819 C/T (OR=4.18 with CI=1.26–13.86) could be considered as risk factors for PCOS...
Source: Meta Gene - Category: Genetics & Stem Cells Source Type: research