Reality of Single Circulating Tumor Cell Sequencing for Molecular Diagnostics in Pancreatic Cancer

To understand the potential and limitations of circulating tumor cell (CTC) sequencing in the context of molecular diagnostics, we investigated the feasibility of identifying the ubiquitous KRAS mutation in single CTCs from pancreatic cancer (PC) patients. We used the NanoVelcro/laser capture microdissection CTC platform, combined with whole genome amplification and KRAS Sanger sequencing. We assessed both our ability to obtain KRAS codon-12 coverage and the degree that allele dropout during whole genome amplification affected the detection of KRAS mutations from single CTCs.

Source: Journal of Molecular Diagnostics - June 29, 2016 Category: Pathology Authors: Colin M. Court, Jacob S. Ankeny, Shonan Sho, Shuang Hou, Qingyu Li, Carolyn Hsieh, Min Song, Xinfang Liao, Matthew M. Rochefort, Zev Wainberg, Thomas G. Graeber, Hsian-Rong Tseng, James S. Tomlinson Tags: Regular Article Source Type: research