Diagnostic Detection of Allelic Losses and Imbalances by Next-Generation Sequencing

Cancer cells are genomic unstable and accumulate tumor type –specific molecular aberrations, which may represent hallmarks for predicting prognosis and targets for therapy. Co-deletion of chromosomes 1p and 19q marks gliomas with an oligodendroglioma component and predicts a better prognosis and response to chemotherapy. In the current study, we present a novel method to detect chromosome 1p/19q co-deletion or loss of heterozygosity (LOH) in a diagnostic setting, based on single-nucleotide polymorphism (SNP) analysis and next-generation sequencing (NGS) on an Ion Torrent platform.

Source: Journal of Molecular Diagnostics - July 22, 2016 Category: Pathology Authors: Hendrikus J. Dubbink, Peggy N. Atmodimedjo, Ronald van Marion, Peter H.J. Riegman, Johan M. Kros, Martin J. van den Bent, Winand N.M. Dinjens Tags: Regular Article Source Type: research