Emodin Protects Mice against Radiation-induced Mortality and Intestinal Injury via Inhibition of Apoptosis and Modulation of p53

Publication date: Available online 3 August 2016 Source:Environmental Toxicology and Pharmacology Author(s): Jing Wang, Yue Zhang, Qiuzhen Zhu, Yulan Liu, Hao Cheng, Yuefan Zhang, Tiejun Li The aim of this study was to explore the protective effect of emodin, a plant-derived anthraquinone, against gamma radiation-induced mortality and intestinal injury in mice, and to investigate the radioprotective molecular mechanism. C57BL/6 male mice were pre-treated with emodin for 7days via oral gavage before gamma radiation. We found that pretreatment with emodin prolonged mice survival time after 9Gy total body irradiation (TBI). Mice were sacrificed at 1 week after 7Gy TBI, we found that emodin attenuated intestinal morphological changes and increase villus height, crypt numbers, and reduced villus and crypt apoptosis as well as inhibited the expression of p53. MTT assay, flow cytometry, Hoechst 33258 staining, real-time PCR, and Western blotting indicated that emodin pretreatment can effectively increase Human umbilical venous endothelial cells (HUVECs) viability and attenuate cell apoptosis; it also inhibited the expression of p53, Bax, and Caspase3 in HUVECs after irradiation. In summary, these results suggest the potential of emodin as an effective radioprotectant against radiation-induced intestinal injury.
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research