Abstract < /h3 > < p class= " a-plus-plus " > < em class= " a-plus-plus " > V < /em > - < em class= " a-plus-plus " > set and transmembrane domain < /em > - < em class= " a-plus-plus " > containing 1 < /em > ( < em class= " a-plus-plus " > VSTM1 < /em > ), which is downregulated in bone marrow cells from leukemia patients, may provide a diagnostic and treatment targe..."> Abstract < /h3 > < p class= " a-plus-plus " > < em class= " a-plus-plus " > V < /em > - < em class= " a-plus-plus " > set and transmembrane domain < /em > - < em class= " a-plus-plus " > containing 1 < /em > ( < em class= " a-plus-plus " > VSTM1 < /em > ), which is downregulated in bone marrow cells from leukemia patients, may provide a diagnostic and treatment targe..." /> Abstract < /h3 > < p class= " a-plus-plus " > < em class= " a-plus-plus " > V < /em > - < em class= " a-plus-plus " > set and transmembrane domain < /em > - < em class= " a-plus-plus " > containing 1 < /em > ( < em class= " a-plus-plus " > VSTM1 < /em > ), which is downregulated in bone marrow cells from leukemia patients, may provide a diagnostic and treatment targe..." />

Synergistic antitumor activity of triple-regulated oncolytic adenovirus with VSTM1 and daunorubicin in leukemic cells

< h3 class= " a-plus-plus " > Abstract < /h3 > < p class= " a-plus-plus " > < em class= " a-plus-plus " > V < /em > - < em class= " a-plus-plus " > set and transmembrane domain < /em > - < em class= " a-plus-plus " > containing 1 < /em > ( < em class= " a-plus-plus " > VSTM1 < /em > ), which is downregulated in bone marrow cells from leukemia patients, may provide a diagnostic and treatment target. Here, a triple-regulated oncolytic adenovirus was constructed to carry a < em class= " a-plus-plus " > VSTM1 < /em > gene expression cassette, SG611-VSTM1, and contained the < em class= " a-plus-plus " > E1a < /em > gene with a 24-nucleotide deletion within the CR2 region under control of the human telomerase reverse transcriptase promoter, < em class= " a-plus-plus " > E1b < /em > gene directed by the hypoxia response element, and < em class= " a-plus-plus " > VSTM1 < /em > gene controlled by the cytomegalovirus promoter. Real-time quantitative PCR and Western blot analyses showed that SG611-VSTM1 expressed VSTM1 highly efficiently in the human leukemic cell line K562 compared with SG611. In Cell Counting Kit-8 and flow cytometric assays, SG611-VSTM1 exhibited more potent anti-proliferative and pro-apoptotic effects in leukemic cells compared with SG611 and exerted synergistic cytotoxicity with low-dose daunorubicin (DNR) in vitro. In xenograft models, SG611-VSTM1 intratumorally injected at a dose of 1  × 10 < sup class= " a-plus-plus " > 9 < /sup > plaque forming units c...
Source: Apoptosis - Category: Molecular Biology Source Type: research