Strategic siRNA Screening Approaches to Target Cancer at the Cancer Research UK Beatson Institute.

Strategic siRNA Screening Approaches to Target Cancer at the Cancer Research UK Beatson Institute.
Comb Chem High Throughput Screen. 2014 Mar 23;
Authors: Shanks EJ
Abstract
The RNAi Screening Facility at the Cancer Research UK Beatson Institute combines siRNA genome-wide screening with drug screening coupled with High Content Imaging and fluorescence-based phenotypic assays to target multiple types of cancer. Here, we describe the infrastructure of the Facility and the approaches we utilise. We also share our experiences in running such a facility and developing and executing screening campaigns, with particular regard to high content multiparametric analysis, data management and statistical analysis.
PMID: 24661213 [PubMed - as supplied by publisher]

Source: Combinatorial Chemistry and High Throughput Screening - March 23, 2014 Category: Chemistry Authors: Shanks EJ Tags: Comb Chem High Throughput Screen Source Type: research