Performance of four different agar plate methods for rectal swabs, synergy disk tests, and MBL-Etest for clinical isolates in detecting carbapenemase-producing Klebsiella pneumoniae.

Performance of four different agar plate methods for rectal swabs, synergy disk tests, and MBL-Etest for clinical isolates in detecting carbapenemase-producing Klebsiella pneumoniae.
J Med Microbiol. 2016 Jul 19;
Authors: Papadimitriou-Olivgeris M, Vamvakopoulou S, Spyropoulou Α, Bartzavali C, Marangos M, Anastassiou ED, Spiliopoulou I, Christofidou M
Abstract
The aim of the study was to compare four different agar plate methods in the identification of carbapenemase-producing Klebsiella pneumoniae (CP-Kp) from rectal samples and to assess the role of phenotypic methodologies in the identification of carbapenemase type from clinical K. pneumoniae isolates. Two chromogenic agars (Brilliance CRE, CHROMagar KPC) were compared to MacConkey agar plates with ertapenem or imipenem disks for the identification of carbapenemase-producing Klebsiella pneumoniae (CP-Kp) from 912 rectal swabs. CP-Kp was detected in 329 samples by either agar methodology (299 KPC-positive, 27 VIM-positive, three KPC- and VIM-positive). Sensitivity of Brilliance CRE, CHROMagar KPC, MacConkey agar plus imipenem or ertapenem disk (inhibition zone <25mm) was 96.8%, 99.2%, 67.2% and 81.8%, whereas, specificity was 90.9%, 78.2%, 98.1% and 97.9%, respectively. Synergy meropenem-disk tests with EDTA or phenylboronic acid were used in order to detect the carbapenemase type as compared to PCR results (blaVIM, blaKPC, blaNDM) from 2515 isolates with reduced susceptibilit...

Source: Journal of Medical Microbiology - July 18, 2016 Category: Microbiology Authors: Papadimitriou-Olivgeris M, Vamvakopoulou S, Spyropoulou Α, Bartzavali C, Marangos M, Anastassiou ED, Spiliopoulou I, Christofidou M Tags: J Med Microbiol Source Type: research