Comparison of multiplex real-time PCR and PCR-reverse blot hybridization assay for the direct and rapid detection of bacteria and antibiotic resistance determinants in positive culture bottles.

Comparison of multiplex real-time PCR and PCR-reverse blot hybridization assay for the direct and rapid detection of bacteria and antibiotic resistance determinants in positive culture bottles. J Med Microbiol. 2016 Jul 21; Authors: Wang HY, Kim S, Kim J, Park SD, Kim HY, Uh Y, Lee H Abstract The aim of this study was to evaluate the performance of a commercially available multiplex real-time PCR assay and a PCR-reverse blot hybridization assay (REBA) for the rapid detection of bacteria and identification of antibiotic resistance genes directly from blood culture bottles and to compare the results of these molecular assays with conventional culture methods. The molecular diagnostic methods were used to evaluate 593 blood culture bottles from patients with BSIs. The detection positivity of multiplex real-time PCR assay for gram-positive bacteria, gram-negative bacteria, and Candida spp. was equivalent to PCR-REBA as 99.6 %, 99.1 %, and 100 %, respectively. Using conventional bacterial cultures as the gold standard, the sensitivity, specificity, positive predictive value, and negative predictive value of these two molecular methods were 99.5 % (95% confidence interval [CI] 0.980-1.0, p<0.001), 100 % (95% CI 0.983-1.0, p<0.001), 100 %, and 99 %, respectively. However, positivity of the Real-MRSA multiplex real-time PCR assay targeting the mecA gene to detect methicillin resistance was lower than that of the PCR-REBA method, detect...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research