Tideglusib induces apoptosis in Human Neuroblastoma IMR32 cells, provoking sub-G0/G1 accumulation and ROS generation

Publication date: Available online 20 July 2016 Source:Environmental Toxicology and Pharmacology Author(s): Theodore Lemuel Mathuram, Vilwanathan Ravikumar, Lisa M. Reece, Selvaraju Karthik, Changam Sheela Sasikumar, Kotturathu Mammen Cherian Neuroblastoma is the most common tumor amongst children amounting to nearly 15% of cancer deaths. This cancer is peculiar in its characteristics, exhibiting differentiation, maturation and metastatic transformation leading to poor prognosis and low survival rates among children. Chemotherapy, though toxic to normal cells, has shown to improve the survival of the patient with emphasis given more towards targeting angiogenesis. Recently, Tideglusib was designed as an ‘Orphan Drug’ to target the neurodegenerative Alzheimer’s disease and gained significant momentum in its function during clinical trials. Duffy et al. recently reported a reduction in cell viability of human IMR32 neuroblastoma cells when treated with Tideglusib at varying concentrations. We investigated the effects of Tideglusib, at various concentrations, compared to lithium chloride at various concentrations, on IMR32 cells. Lithium, a known GSK-3 inhibitor, was used as a standard to compare the efficiency of Tideglusib in a dose-dependent manner. Cell viability was assessed by MTT assay. The stages of apoptosis were evaluated by AO/EB staining and nuclear damage was determined by Hoechst 33258 staining. Reactive oxygen species (ROS) and mitochondrial ...
Source: Environmental Toxicology and Pharmacology - Category: Environmental Health Source Type: research