Abstract < /h3 > < p class= " a-plus-plus " > The purpose of this study was to evaluate a novel drug delivery system comprised of ferric-cobalt electro-magnetic nano-material (CoFe2O4@ BaTiO3; MENP) bound to siRNA targeting Beclin1 (MENP-siBeclin1) to cross the blood –brain barrier (BBB) and attenuate the neurotoxic effects of HIV-1 infection in the central nervous ..."> Abstract < /h3 > < p class= " a-plus-plus " > The purpose of this study was to evaluate a novel drug delivery system comprised of ferric-cobalt electro-magnetic nano-material (CoFe2O4@ BaTiO3; MENP) bound to siRNA targeting Beclin1 (MENP-siBeclin1) to cross the blood –brain barrier (BBB) and attenuate the neurotoxic effects of HIV-1 infection in the central nervous ..." /> Abstract < /h3 > < p class= " a-plus-plus " > The purpose of this study was to evaluate a novel drug delivery system comprised of ferric-cobalt electro-magnetic nano-material (CoFe2O4@ BaTiO3; MENP) bound to siRNA targeting Beclin1 (MENP-siBeclin1) to cross the blood –brain barrier (BBB) and attenuate the neurotoxic effects of HIV-1 infection in the central nervous ..." />

Electro-Magnetic Nano-Particle Bound Beclin1 siRNA Crosses the Blood –Brain Barrier to Attenuate the Inflammatory Effects of HIV-1 Infection in Vitro

< h3 class= " a-plus-plus " > Abstract < /h3 > < p class= " a-plus-plus " > The purpose of this study was to evaluate a novel drug delivery system comprised of ferric-cobalt electro-magnetic nano-material (CoFe2O4@ BaTiO3; MENP) bound to siRNA targeting Beclin1 (MENP-siBeclin1) to cross the blood –brain barrier (BBB) and attenuate the neurotoxic effects of HIV-1 infection in the central nervous system following on-demand release of siRNA using an in vitro primary human BBB model. Beclin1 is a key protein in the regulation of the autophagy pathway and we have recently demonstrated the impor tance of Beclin1 in regulating viral replication and viral-induced inflammation in HIV-1-infected microglia. The MENP-siBeclin1 nano-formulation did not compromise the physiological function or integrity of the BBB model. Furthermore, the in vitro BBB data revealed that MENP-siBeclin1 could efficien tly attenuate viral replication and viral-induced inflammation, likely due to STAT1/ NF-κB signaling pathways. MENP-siBeclin1 also silenced Beclin1 protein expression in HIV-1-infected microglial cells within the model system. In addition, the cytotoxic effects of direct treatment with siBeclin1 an d MENP alone or in nano-formulation on primary human neuronal cells showed a minimal amount of cell death. Overall, the data shows that the nano-formulation can silence the < em class= " a-plus-plus " > BECN1 < /em > gene as an effective mechanism to attenuate HIV-1 replication and viral-induced i...
Source: Journal of NeuroImmune Pharmacology - Category: Drugs & Pharmacology Source Type: research