ABCA siRNA Transfection of Neurons & Astrocytes

In this study, Dr. Marina Guizzetti and his team use our < a href= " http://www.neuromics.com/ittrium/visit/A1x66x1y1x85b1x1x9cy1x622fx1x96y1x351dx1x82 " target= " _blank " > i-Fect Kit < /a > and several other commercially available kits to knockdown ABCG1 and ABCG4: < a href= " http://www.sciencedirect.com/science/article/pii/S1388198112002041 " target= " _blank " > Jing Chen, Xiaolu Zhang, Handojo Kusumo, Lucio G. Costa, c, Marina Guizzettia. Cholesterol efflux is differentially regulated in neurons and astrocytes: Implications for brain cholesterol homeostasis. < /a > Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1831 (2), p.263-275, Feb 2013. doi:10.1016/j.bbalip.2012.09.007. < br / > < strong > Abstract: < /strong > Disruption of cholesterol homeostasis in the central nervous system (CNS) has been associated with neurological, neurodegenerative, and neurodevelopmental disorders. The CNS is a closed system with regard to cholesterol homeostasis, as cholesterol-delivering lipoproteins from the periphery cannot pass the blood –brain-barrier and enter the brain. Different cell types in the brain have different functions in the regulation of cholesterol homeostasis, with astrocytes producing and releasing apolipoprotein E and lipoproteins, and neurons metabolizing cholesterol to 24(S)-hydroxycholesterol. We present evide nce that astrocytes and neurons adopt different mechanisms also in regulating cholesterol efflux. We found that in astr...

Source: siRNA and DsiRNA Transfection Efficiency - November 17, 2012 Category: Neuroscience Tags: ABCA1 i-Fect siRNA Delivery in vitro siRNA Transfection Efficiency siRNA transfection of neurons siRNA trasfection of astrocytes Source Type: news