Countrywide dissemination of a DHA-1-type plasmid-mediated AmpC β-lactamase-producing K. pneumoniae ST11 international high-risk clone in Hungary, 2009-2013.

Countrywide dissemination of a DHA-1-type plasmid-mediated AmpC β-lactamase-producing K. pneumoniae ST11 international high-risk clone in Hungary, 2009-2013. J Med Microbiol. 2016 Jul 1; Authors: Kis Z, Tóth Á, Jánvári L, Damjanova I Abstract The first plasmid-mediated AmpC β-lactamase producing K. pneumoniae (pAmpC KP) isolate was detected in December 2009 in Hungary. Hungarian microbiological laboratories were asked to send all KP strains showing cefoxitin resistance and decreased susceptibility or resistance to any 3rd generation cephalosporins to the Reference Laboratories at the National Center for Epidemiology. Investigation was conducted in order to outline spatio-temporal distribution and genetic characterization of pAmpC KP isolates in Hungary. Between December 2009 and December 2013, 312 consecutive KP clinical isolates were confirmed to produce pAmpCs. All isolates showed resistance to 3rd generation cephalosporins, aminoglycosides, and fluoroquinolones and 77% were non-susceptible to at least one carbapenem. Analysis of β-lactamase genes showed blaDHA-1 in all and additionally blaCTX-M-15 in 90% of isolates. PFGE typing revealed twelve pulsotypes; of these KP053 (262/312) and KP070 (38/312) belonged to sequence type ST11 and comprised 96% of the isolates. The blaDHA-1 and blaCTX-M-15 co-producing KP053/ST11 clone affected 234 patients and spread to 55 health care centers across Hungary during the study period. Thre...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research