Crystallization and preliminary X-ray crystallographic analysis of PBPD2 from Listeria monocytogenes

In this study, PBPD2, a low-molecular-weight PBP encoded by lmo2812 from Listeria monocytogenes, was overexpressed in Escherichia coli, purified and crystallized at 295 K using the sitting-drop vapour-diffusion method. The crystal belonged to the primitive orthorhombic space group P212121, with unit-cell parameters a = 37.7, b = 74.7, c = 75.1 Å, and diffracted to 1.55 Å resolution. There was one molecule in the asymmetric unit. The preliminary structure was determined by the molecular-replacement method.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: penicillin-binding proteins PBPD2 Listeria monocytogenes crystallization communications Source Type: research