Antibody-guided vaccine design: identification of protective epitopes.

Antibody-guided vaccine design: identification of protective epitopes. Curr Opin Immunol. 2016 Jun 22;41:62-67 Authors: Lanzavecchia A, Frühwirth A, Perez L, Corti D Abstract In the last decade, progress in the analysis of the human immune response and in the isolation of human monoclonal antibodies have provided an innovative approach to the identification of protective antigens which are the basis for the design of vaccines capable of eliciting effective B-cell immunity. In this review we illustrate, with relevant examples, the power of this approach that can rapidly lead to the identification of protective antigens in complex pathogens, such as human cytomegalovirus and Plasmodium falciparum, and of conserved sites in highly variable antigens, such as influenza hemagglutinin and HIV-1 Env. We will also discuss how the genealogical analysis of antigen-stimulated B cell clones provides the basis to delineate the best suitable prime-boost vaccination strategy for the induction of broadly neutralizing antibodies. PMID: 27343848 [PubMed - as supplied by publisher]
Source: Current Opinion in Immunology - Category: Allergy & Immunology Authors: Tags: Curr Opin Immunol Source Type: research

Related Links:

We report that electrical stimulation (ES) stimulation of post-stroke aged rats led to an improved functional recovery of spatial long-term memory (T-maze), but not on the rotating pole or the inclined plane, both tests requiring complex sensorimotor skills. Surprisingly, ES had a detrimental effect on the asymmetric sensorimotor deficit. Histologically, there was a robust increase in the number of doublecortin-positive cells in the dentate gyrus and SVZ of the infarcted hemisphere and the presence of a considerable number of neurons expressing tubulin beta III in the infarcted area. Among the genes that were unique...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Over a lifetime, the burden of infectious disease - and particularly persistent infections such as cytomegalovirus - influences the pace of aging via its detrimental impact on immune function in later life. The slow upward trend in life expectancy over the past two centuries is due in no small part to reductions in infectious disease that accompanied improvements in sanitation and then medicine. In addition, in old age, once the immune system declines into ineffectieness, infectious disease becomes a much more serious concern. Infections that a young person defeats with ease become life-threatening. The primary strategy to...
Source: Fight Aging! - Category: Research Authors: Tags: Medicine, Biotech, Research Source Type: blogs
In conclusion, DNA vaccination with pVAX1-H9 and pCR-H9 could protect turkey from the H9N2 virus, but vaccination regimes need to be improved. PMID: 32663531 [PubMed - as supplied by publisher]
Source: Journal of Virological Methods - Category: Virology Authors: Tags: J Virol Methods Source Type: research
Purpose of review Studying primary immunodeficiencies (PIDs) provides insights into human antiviral immunity in the natural infectious environment. This review describes new PIDs with genetic defects that impair innate antiviral responses. Recent findings New genetic defects in the interferon (IFN) signaling pathway include IFNAR1 deficiency, which causes uncontrolled infections with measles-mumps-rubella or yellow fever vaccines, and possibly also cytomegalovirus (CMV); and IRF9 deficiency, which results in influenza virus susceptibility. Genetic defects in several pattern recognition receptors include MDA5 deficienc...
Source: Current Opinion in Pediatrics - Category: Pediatrics Tags: ALLERGY, IMMUNOLOGY AND RELATED DISORDERS: Edited by Jordan S. Orange Source Type: research
The TWiVologists consider whether to receive an influenza vaccine in August (in the northern hemisphere), and mice implanted with human lung fragments for studying microbial pathogens. Click arrow to play Download TWiV 563 (55 MB .mp3, 91 min) Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV!
Source: virology blog - Category: Virology Authors: Tags: This Week in Virology BLT mouse coronavirus flu vaccine human cytomegalovirus humanized mouse immunodeficient mice influenza lung implant mouse model vaccine effectiveness viral viruses zika virus Source Type: blogs
Oregon Health&Science University received a grant from the Gates Foundation to develop the Holy Grail of vaccines: a universal flu vaccine. OHSU is one of about six institutions that are splitting up to $12 million through the Grand Challenge for Universal Influenza Vaccine Development. The basis for OHSU’s effort will be its cytomegalovirus platform, which it is already using to develop vaccines against HIV and tuberculosis. The i nfluenza virus would be inserted into the CMV vector to stimulate…
Source: bizjournals.com Health Care:Biotechnology headlines - Category: Biotechnology Authors: Source Type: news
Abstract Celiac disease is a complex autoimmune enteropathy of the small intestine that commonly occurs in genetically predisposed individuals due to intake of gluten and related proteins. Gluten consumption, duration of breast-feeding, various infections, especially frequent intestine infections, vaccinations and use of antibiotics can be linked to celiac disease. It is predicted that it affects 1% of the global population and its incidence rate is increasing. Celiac disease is closely related to Turner's syndrome, type I diabetes mellitus, Down's syndrome, and other autoimmune conditions and most people with the...
Source: Current Pharmaceutical Biotechnology - Category: Biotechnology Authors: Tags: Curr Pharm Biotechnol Source Type: research
The open access editorial noted here serves as an introduction to some of the current thinking on the role of cytomegalovirus (CMV) in the age-related decline of the immune system. CMV infection is pervasive throughout the population, particularly in the old. This persistent viral infection cannot be effectively cleared by the immune system, and an ever greater percentage of immune cells become uselessly specialized to fight CMV. This leaves ever fewer immune cells ready to tackle other threats. This seems an important component of immune dysfunction, one that can perhaps be addressed by selectively destroying these immune...
Source: Fight Aging! - Category: Research Authors: Tags: Daily News Source Type: blogs
In this study, we found that cofilin competes with tau for direct microtubule binding in vitro, in cells, and in vivo, which inhibits tau-induced microtubule assembly. Genetic reduction of cofilin mitigates tauopathy and synaptic defects in Tau-P301S mice and movement deficits in tau transgenic C. elegans. The pathogenic effects of cofilin are selectively mediated by activated cofilin, as active but not inactive cofilin selectively interacts with tubulin, destabilizes microtubules, and promotes tauopathy. These results therefore indicate that activated cofilin plays an essential intermediary role in neurotoxic signaling th...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Mireia Pedragosa1†, Graciela Riera1†, Valentina Casella1, Anna Esteve-Codina2,3, Yael Steuerman4, Celina Seth1, Gennady Bocharov5,6, Simon Heath2,3, Irit Gat-Viks4, Jordi Argilaguet1* and Andreas Meyerhans1,7* 1Infection Biology Laboratory, Department of Experimental and Health Sciences (DCEXS), Universitat Pompeu Fabra, Barcelona, Spain 2CNAG-CRG, Center for Genomic Regulation (CRG), Barcelona Institute of Science and Technology, Barcelona, Spain 3Universitat Pompeu Fabra, Barcelona, Spain 4Cell Research and Immunology Department, Tel Aviv University, Tel Aviv, Israel 5Marchuk Institute of Numeric...
Source: Frontiers in Immunology - Category: Allergy & Immunology Source Type: research
More News: Allergy & Immunology | Cytomegalovirus | Influenza | Influenza Vaccine | Vaccines