Non-adherent spheres with multiple myeloma surface markers contain cells that contribute to the sphere formation and are capable of internalizing extracellular double-stranded DNA

We present phenotypic analysis of free-floating spheres derived from the bone marrow clonogenic aspirate of a multiple myeloma patient. Besides CD73/CD20+/CD45+/CD19dim B cells, these spheres encompass a special subpopulation of cells that can natively internalize extracellular double-stranded DNA and which contribute to the colony assembly. Various cell types found in the free-floating spheres communicate with each other by secreting distinct sets of cytokines. These spheres are capable of forming xenotrasplants upon grafting to immunodeficient NOD/SCID mice.

Source: Clinical Lymphoma, Myeloma and Leukemia - June 22, 2016 Category: Hematology Authors: Evgeniya V. Dolgova, Ekaterina Ya Shevela, Tamara V. Tyrinova, Alexandra M. Minkevich, Anastasia S. Proskurina, Ekaterina A. Potter, Konstantin E. Orishchenko, Evgeniy L. Zavjalov, Sergey I. Bayborodin, Valeriy P. Nikolin, Nelly A. Popova, Natalia V. Pron Tags: Original Study Source Type: research