Vibrio cholerae GbpA elicits necrotic cell death in intestinal cells.

Vibrio cholerae GbpA elicits necrotic cell death in intestinal cells.
J Med Microbiol. 2016 Jun 20;
Authors: Mandal S, Chatterjee NS
Abstract
Vibrio cholerae N-acetylglucosamine binding protein GbpA is a secretory protein which facilitates the initial adherence of bacteria in the human intestine. Until now, considerable progress in the characterization of GbpA has been done, yet little is known about its role in host response. Our present studies demonstrated that, GbpA at the amount secreted in the intestine resulted in decreased cell viability, altered cell morphology, disruption of cell membrane integrity and damage of cellular DNA indicating necrotic cell death. We observed that GbpA exposure leads to mitochondrial dysfunction, characterized by accumulation of ROS, depolarization of mitochondrial membrane potential and depletion of ATP pool in host cells. Additionally, the intracellular ROS, accumulated in response to GbpA was found to induce the migration of NF-ĸB from cytoplasm into nucleus in host cells. Taken together, these results prompted us to conclude that GbpA orchestrate a necrotic response in host cells which may have implications in immune response.
PMID: 27324251 [PubMed - as supplied by publisher]

Source: Journal of Medical Microbiology - June 19, 2016 Category: Microbiology Authors: Mandal S, Chatterjee NS Tags: J Med Microbiol Source Type: research