Emergence and characterization of tigecycline resistance in multidrug-resistant Klebsiella pneumoniae isolates from blood samples of patients in intensive care units in northern China.

Emergence and characterization of tigecycline resistance in multidrug-resistant Klebsiella pneumoniae isolates from blood samples of patients in intensive care units in northern China. J Med Microbiol. 2016 Jun 20; Authors: Xu H, Zhou Y, Zhai X, Du Z, Wu H, Han Y, Huo C, Chen Y Abstract Serious infections in intensive care unit patients caused by multidrug-resistant (MDR) Klebsiella pneumoniae represent a major threat worldwide due to increased mortality and limited treatment options. With the application of tigecycline for MDR pathogens, tigecycline non-susceptible K. pneumoniae isolates have recently emerged in China. To identify the susceptibility profile of MDR K. pneumoniae to tigecycline and evaluate the molecular characterization of tigecycline resistance, 214 MDR K. pneumoniae isolates were collected from blood samples of patients in intensive care unit. Minimum inhibitory concentrations (MICs) and clonal relatedness were determined by standard broth microdilution and multi-locus sequence typing (MLST), respectively. Expression levels of efflux pumps and their global regulators were examined using real-time PCR. Mutations of local repressor were identified by PCR and sequencing. Our results show tigecycline resistance rate of 214 MDR K. pneumoniae isolates was 6.07%. ST11 was the predominant clone type of tigecycline non-susceptible K. pneumoniae isolates. Expression of efflux pump AcrB and global regulator RamA correlated wi...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research