Interactions of diamines with adenosine-5'-triphosphate (ATP) in the systems including copper(II) ions.

Interactions of diamines with adenosine-5'-triphosphate (ATP) in the systems including copper(II) ions.
J Inorg Biochem. 2016 Jun 4;
Authors: Bregier-Jarzebowska R, Gasowska A, Hoffmann SK, Lomozik L
Abstract
Interactions were studied in the systems ATP/tn and ATP/Put (tn=1,3-diaminopropane, Put=putrescine) whereas the complexation reactions in ternary systems Cu(II)/ATP/tn and Cu(II)/ATP/Put. Results of the potentiometric and spectroscopic studies evidenced the formation of adducts of the type (ATP)Hx(PA), where PA=diamine. The thermodynamic stability of the complexes and the mode of interactions were determined. On the basis of analysis of changes in the positions of NMR signals, in the pH range of (ATP)H3(Put) formation, the preferred centres of the interaction between ATP and Put are the endocyclic nitrogen atoms from the nucleotide. On the other hand, the shorter diamine tn in the entire pH range reacts with the phosphate groups from ATP. The positive centres of noncovalent interactions are the protonated NHx(+) groups from amines. In both complexes Cu(ATP)H2(tn) and Cu(ATP)H3(Put) formed in ternary systems at pH<6.5, the amines are in the outer sphere of coordination with the noncovalent interaction with anchoring Cu(ATP). Only the phosphate groups from the nucleotide take part in metalation. At higher pH in the range of Cu(ATP)(PA) complex formation, significant differences in the reactions of the two amines appear. The sho...

Source: Journal of Inorganic Biochemistry - June 3, 2016 Category: Biochemistry Authors: Bregier-Jarzebowska R, Gasowska A, Hoffmann SK, Lomozik L Tags: J Inorg Biochem Source Type: research