Phytogenic synthesis of silver nanoparticles, optimization and evaluation of In vitro antifungal activity against human and plant pathogens

Publication date: Available online 8 June 2016 Source:Microbiological Research Author(s): P. Balashanmugam, M.D. Balakumaran, R. Murugan, K. Dhanapal, P.T. Kalaichelvan An attempt was made to synthesis of biocompatible silver nanoparticles from ten different Cassia sp. Among them, C. roxburghii aqueous leaf extract supported the synthesis of highly efficient and stable AgNPs. The synthesis of AgNPs was optimized at different physico-chemical condition and highly stable AgNPs were synthesized with 1. 0mL of C. roxburghii leaf extract, pH 7.0, 1.0mM AgNO3 and at 37°C. The synthesized AgNPs were characterized by XPS, DLS and ZETA potential. DLS and ZETA potential analysis, the average AgNPs size was 35nm and the zeta potential was −16.0mV. The AgNPs exhibit higher antifungal activity when compared with the conventional antifungal drug amphotericin B against all the tested human fungal pathogens such as Aspergillus niger, A. fumigatus, A. flavus, Penicillium sp., C. albicans and the plant pathogens such as R. solani, F. oxysporum and Curvularia sp. TEM analysis showed distinct structural changes in the cell membranes of C. albicans upon AgNPs treatment. These results suggest that phytosynthesized AgNPs could be used as effective growth inhibitors in controlling various human and plant diseases caused by fungi.

Source: Microbiological Research - June 8, 2016 Category: Infectious Diseases Source Type: research