Global Tn‐seq Analysis of Carbohydrate Utilization and Vertebrate Infectivity of Borrelia burgdorferi

This article is protected by copyright. All rights reserved. Abbreviated Summary This work establishes Tn‐seq as a new tool for performing high‐throughput genetic fitness analyses in Borrelia burgdorferi. We used the technique to perform an in vitro screen exploring mechanisms of mannose, maltose, trehalose, and N‐acetyl‐glucosamine metabolism in B. burgdorferi as well as a complementing in vivo screen in mice. In doing so we obtained insight into the potential functions of genes annotated as encoding carbohydrate transporters and an additional function of the response regulator Rrp1.

http://onlinelibrary.wiley.com/resolve/doi?DOI=10.1111%2Fmmi.13437

Source: Molecular Microbiology - June 8, 2016 Category: Microbiology Authors: Erin B. Troy, Tao Lin, Lihui Gao, David W. Lazinski, Maureen Lundt, Andrew Camilli, Steven J. Norris, Linden T. Hu Tags: Research Article Source Type: research