Identification and genetic manipulation of human and mouse oesophageal stem cells

Conclusions
We developed clonogenic and organotypic culture systems for the quantitative analyses of human and mouse oesophageal stem/progenitor cells and identified novel cell surface marker combinations that enrich for these cells. Using this system, we demonstrate that elimination of p63 inhibits self-renewal of human oesophageal stem/progenitor cells. We anticipate that these esophagosphere culture systems will facilitate studies of oesophageal stem cell biology and may prove useful for ex vivo expansion of human oesophageal stem cells.

Source: Gut - June 2, 2016 Category: Gastroenterology Authors: Jeong, Y., Rhee, H., Martin, S., Klass, D., Lin, Y., Nguyen, L. X. T., Feng, W., Diehn, M. Tags: Oesophagus Source Type: research