Detection of blaSPM-1, blaKPC, blaTEM and blaCTX-M genes in isolates of Pseudomonas aeruginosa, Acinetobacter spp. and Klebsiella spp. from cancer patients with healthcare associated infections.

Detection of blaSPM-1, blaKPC, blaTEM and blaCTX-M genes in isolates of Pseudomonas aeruginosa, Acinetobacter spp. and Klebsiella spp. from cancer patients with healthcare associated infections. J Med Microbiol. 2016 May 23; Authors: Jácome PR, Alves LR, Jácome-Júnior AT, Silva MJ, Lima JL, Araújo PS, Lopes AC, Maciel MA Abstract Pseudomonas aeruginosa, Acinetobacter spp. and Klebsiella spp. are three of the pathogens most frequently involved in cancer patients infections, and the production of β-lactamases is a major mechanism of resistance due to its wide diversity of existing enzymes. Therefore, the aim of the present study was to investigate the microbiological profile and data related to patients and infections, and search for the some β-lactamases genes in bacterial isolates from hospitalized cancer patients in a hospital in Recife, in Pernambuco/Brazil. A total of 169 isolates were recovered between 2012 and 2014, of which 58 were P. aeruginosa, 36 were Acinetobacter spp. and 75 were Klebsiella spp.. A high percentage of carbapenem resistance was observed in P. aeruginosa and Acinetobacter spp.. Among the carbapenem-resistant bacteria, the blaSPM-1 gene was detected in P. aeruginosa (35.5%) and Acinetobacter spp. (3.8%), while blaKPC was detected in P. aeruginosa (25.8%) only. Among the third and fourth generation cephalosporin resistant strains, in Klebsiella spp. were detected the genes blaTEM (30.6%), blaCTX-M (58.3%)...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research