Evaluation and quantitative analysis of different growth periods of herb–arbor intercropping systems using HPLC and UV–vis methods coupled with chemometrics

In this study, we employed high-performance liquid chromatography (HPLC) and ultraviolet–visible spectroscopy (UV–vis) combined with chemometrics for determination and qualitative evaluation of several kinds of intercropping system with Gentiana rigescens Franch. ex Hemsl. (GR), which is used as an hepatic protector in local communities in China. Results revealed that GR in a Camellia sinensis intercropping system contained most gentiopicroside, sweroside, and total active constituents (six chemical indicators), whose content reached 91.09 ± 3.54, 1.03 ± 0.06, and 104.05 ± 6.48 mg g−1, respectively. The two applied quantitative and qualitative methods reciprocally verified that GR with 2 years of growth period performed better in terms of quality than 1 year, collectively.
Source: Journal of Natural Medicines - Category: Drugs & Pharmacology Source Type: research