Adipose derived mesenchymal stem cells express keratinocyte lineage markers in a co-culture model.

Adipose derived mesenchymal stem cells express keratinocyte lineage markers in a co-culture model. Cell Mol Biol (Noisy-le-grand). 2016;62(5):44-54 Authors: Irfan-Maqsood M, Matin MM, Heirani-Tabasi A, Bahrami M, Naderi-Meshkin H, Mirahmadi M, Hassanzadeh H, Sanjar Moussavi N, Raza-Shah H, Raeesolmohaddeseen M, Bidkhori H, Bahrami AR Abstract Cutaneous wound healing is a complex type of biological event involving proliferation, differentiation, reprograming, trans/de-differentiation, recruitment, migration, and apoptosis of a number of cells (keratinocytes, fibroblasts, endothelial cells, nerve cells and stem cells) to regenerate a multi-layered tissue that is damaged by either internal or external factors. The exact regeneration mechanism of damaged skin is still unknown but the epithelial and other kinds of stem cells located in skin play crucial roles in the healing process. In this work, a co-culture model composed of adipose derived mesenchymal stem cells and keratinocytes was developed to understand the cellular differentiation behaviour in wound healing. Human mesenchymal stem cells were isolated from waste lipoaspirates. Keratinocytes were isolated from neonatal rats skin as well from human adult skin. Both types of cells were cultured and their culturing behaviour was observed microscopically under regular intervals of time. The identity of both cells was confirmed by flow cytometry and qRT-PCR. Cells were co-cultured under ...
Source: Cellular and Molecular Biology - Category: Molecular Biology Tags: Cell Mol Biol (Noisy-le-grand) Source Type: research