Whole genome sequencing-based molecular epidemiologic analysis of autochthonous dengue virus type 1 strains circulating in Japan in 2014.

Whole genome sequencing-based molecular epidemiologic analysis of autochthonous dengue virus type 1 strains circulating in Japan in 2014. Jpn J Infect Dis. 2016 May 9; Authors: Tajima S, Nakayama E, Kotaki A, Moi ML, Ikeda M, Yagasaki K, Saito Y, Shibasaki KI, Saijo M, Takasaki T Abstract Cases of autochthonous dengue virus type 1 (DENV-1) infections were detected in Japan following a 70-year period devoid of dengue outbreaks. We have previously shown that E gene sequences are identical in 11 of 12 DENV-1 strains autochthonous to Japan. However, the E sequence represents only 14% of the genome. In the present study, we sequenced the entire genome of 6 autochthonous DENV-1 strains isolated from patients during the 2014 outbreak. Sequencing of 5 Yoyogi group strains with identical E sequences and 1 Shizuoka strain with a different E sequence revealed that the first Yoyogi group strain identified differed from the Shizuoka strain by 18 amino acid residues and that 2 Yoyogi group strains had different nucleotide sequences whilst the other 3 had identical sequences. Phylogenetic analyses indicated that the Hyogo strain, a Yoyogi group strain, was the first to diverge from the other 4 Yoyogi group strains. The E gene sequence of Yoyogi group strains exhibits the highest homology to those of strains isolated in Malaysia and Singapore during 2013-2014. The patient infected with the Hyogo strain had visited Malaysia before the onset of dengue...
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research