GSE81449 Identification of Beef Heifers with Superior Uterine Capacity for Pregnancy

Contributors : Thomas W Geary ; Gregory W Burns ; Joao Moraes ; James I Moss ; Anna C Denicol ; Kyle B Dobbs ; M S Ortega ; Peter J Hansen ; Michael E Wehrman ; Holly L Neibergs ; Eleanore V O'Neil ; Sustanta K Behura ; Thomas E SpencerSeries Type : Expression profiling by high throughput sequencingOrganism : Bos taurusInfertility and subfertility represent major problems in domestic animals and humans, and the majority of embryonic loss occurs during the first month of gestation that involves pregnancy recognition and conceptus implantation. The critical genes and physiological pathways in the endometrium that mediate pregnancy establishment and success are not well understood. In Study One, 270 predominantly Angus heifers were classified based on fertility using four rounds of serial embryo transfer (ET) to select animals with intrinsic differences in pregnancy loss. In each round, a single in vitro-produced high-quality embryo was transferred into heifers on day 7 post-estrus and pregnancy was determined on days 28 and 42 by ultrasound and then terminated. Heifers were classified based on pregnancy success as high fertile (HF), subfertile (SF), or infertile (IF). In Study Two, fertility-classified heifers were resynchronized and bred with semen from a single high fertility bull. Blood samples were collected every other day from days 0 to 36 post-mating. Pregnancy rate was determined on day 28 by ultrasound and tended to be higher in HF (70.4%) and SF (46.7%) than IF (0%) ...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Bos taurus Source Type: research