Macrolide, glycopeptide resistance and virulence genes in Enterococcus species isolates from dairy cattle.

Macrolide, glycopeptide resistance and virulence genes in Enterococcus species isolates from dairy cattle. J Med Microbiol. 2016 May 10; Authors: Iweriebor BC, Obi LC, Okoh AI Abstract The genus Enterococcus is known to possess the capacity to acquire and disseminate antimicrobial resistant determinants alongside the ability to produce various virulence genes that enables it to establish infections. We assessed the prevalence and antibiogram profiles of Enterococcus spp. in feacal samples of dairy cattle. Fecal swab samples were collected from 400 dairy cattle from two commercial cattle farms in two rural communities in the Eastern Cape, South Africa. Confirmation of enterococci isolates was carried out by polymerase chain reaction (PCR) targeting the tuf gene. Species delineation was by species-specific primers targeting the superoxide dismutase (sodA) gene in a multiplex PCR assay. Isolates were screened for the presence of virulence genes while antimicrobial resistance determinants to erythromycin, vancomycin, and streptomycin were evaluated molecularly. A total of 340 isolates were confirmed as belonging to the genus Enterococcus. Species distribution among the isolates consisted of E. faecium (52.94%) and E.durans (23.53%) in preponderance compared to the three other species namely E. faecalis (8.8%), E. hirae (8.6%) and E. casseliflavus (5.9%). All were resistant to vancomycin while 99% showed resistance to aminoglycoside and 9...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research