Construction and characterization of a cigR deletion mutant of Salmonella enterica serovar Pullorum.

Construction and characterization of a cigR deletion mutant of Salmonella enterica serovar Pullorum. Avian Pathol. 2016 May 10;:1-22 Authors: Yin J, Xia J, Tao M, Xu L, Li Q, Geng S, Jiao X Abstract Salmonella enterica serovar Pullorum (S. Pullorum) is the causative agent of pullorum disease (PD) and results in severe economic losses to the poultry industry. As a Salmonella type III secretion system 2 (T3SS2) effector and predicted membrane protein, CigR is encoded by cigR gene within Salmonella pathogenicity island 3 (SPI3). In order to research the influence of the cigR gene on S. Pullorum, a cigR mutant of S. Pullorum S06004 was constructed by lambda Red recombination system, and then its characterization were analyzed. Lack of cigR did not affect the growth and biochemical properties, but resulted in decreased biofilm formation, the mutant strain was stable with the deletion of cigR gene. Macrophage infection assay and in vivo competition assay showed that the mutant strain increased the replication and/or survival ability in HD11 cell line and in chickens compared to that of the parent strain, the half-lethal dose (LD50) of the mutant strain was one fifth of the parent strain for 2-day-old chickens when injected intramuscularly. These results demonstrate CigR plays roles in biofilm formation and pathogenicity of S. Pullorum, deletion of cigR can decrease significantly biofilm formation and increase significantly virulence. ...
Source: Avian Pathology - Category: Pathology Authors: Tags: Avian Pathol Source Type: research