Structural and functional insights into the stationary-phase survival protein SurE, an important virulence factor of Brucella abortus

The stationary-phase survival protein SurE from Brucella abortus (BaSurE) is a metal-dependent phosphatase that is essential for the survival of this bacterium in the stationary phase of its life cycle. Here, BaSurE has been biochemically characterized and its crystal structure has been determined to a resolution of 1.9 Å. BaSurE was found to be a robust enzyme, showing activity over wide ranges of temperature and pH and with various phosphoester substrates. The active biomolecule is a tetramer and each monomer was found to consist of two domains: an N-terminal domain, which forms an approximate α + β fold, and a C-terminal domain that belongs to the α/β class. The active site lies at the junction of these two domains and was identified by the presence of conserved negatively charged residues and a bound Mg2+ ion. Comparisons of BaSurE with its homologues have revealed both common features and differences in this class of enzymes. The number and arrangement of some of the equivalent secondary structures, which are seen to differ between BaSurE and its homologues, are responsible for a difference in the size of the active-site area and the overall oligomeric state of this enzyme in other organisms. As it is absent in mammals, it has the potential to be a drug target.
Source: Acta Crystallographica Section F - Category: Biochemistry Authors: Tags: stationary-phase survival protein nucleotidase Brucella abortus phagosome domain swapping Rossmann fold malachite green assay research communications Source Type: research

Related Links:

Publication date: Available online 27 March 2020Source: Journal of Hospital InfectionAuthor(s): M.E. Elsayed, O.M. Williams
Source: Journal of Hospital Infection - Category: Infectious Diseases Source Type: research
Publication date: Available online 25 March 2020Source: Veterinary MicrobiologyAuthor(s): Mingxing Tian, Yi Yin, Zhengmin Lian, Zichen Li, Meiying Song, Hai Hu, Xiang Guan, Chan Ding, Shaohui Wang, Tao Li, Jingjing Qi, Shengqing Yu
Source: Veterinary Microbiology - Category: Veterinary Research Source Type: research
Despite the considerable efforts made to address the issue of brucellosis worldwide, its prevalence in dairy products continues to be difficult to estimate and represents a key public health issue around the w...
Source: BMC Infectious Diseases - Category: Infectious Diseases Authors: Tags: Research article Source Type: research
Brucellosis has been reported in several regions of Hainan Province, but the extent of the disease has not been fully elucidated. Conventional biotyping methods, multiple locus variable number tandem repeats analysis (MLVA), and single-nucleotide polymorphisms (SNPs) from draft genome sequencing were employed to characterize the strains. There were four biovars (Brucella melitensis bv. 1, 2, and 3 and Brucella suis bv. 3) detected, which showed that the biovar diversity of Brucella in Hainan is higher than in other areas of China. Both B. melitensis bv. 3 and B. suis bv. 3 were dominant species and showed epidemiology patt...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
In this study, the sensitivity and diagnostic accuracy of B. henselae SA2 WB were higher than those of B. henselae SA2 IFA testing. Some B. henselae SA2 immunodominant proteins were recognized by dogs experimentally and naturally infected with Bartonella spp. other than B. henselae. Additional research is necessary to more fully define the utility of WB for the serodiagnosis of canine bartonelloses.
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Clinical Veterinary Microbiology Source Type: research
The original article [1] incorrectly presents final author, Eugene H. Johnson ’s name incorrectly whereby middle initial, ‘H.’ is mistakenly presented as a Family Name.
Source: BMC Veterinary Research - Category: Veterinary Research Authors: Tags: Correction Source Type: research
Osteoarticular disease is a frequent complication of human brucellosis. Vaccination remains a critical component of brucellosis control, but there are currently no vaccines for use in humans, and no in vitro models for assessing the safety of candidate vaccines in reference to the development of bone lesions currently exist. While the effect of Brucella infection on osteoblasts has been extensively evaluated, little is known about the consequences of osteoclast infection. Murine bone marrow-derived macrophages were derived into mature osteoclasts and infected with B. abortus 2308, the vaccine strain S19, and attenuated mut...
Source: Infection and Immunity - Category: Infectious Diseases Authors: Tags: Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Source Type: research
Publication date: Available online 19 March 2020Source: Comparative Immunology, Microbiology and Infectious DiseasesAuthor(s): Elham Mohammadi, Mehdi Golchin
Source: Comparative Immunology, Microbiology and Infectious Diseases - Category: Infectious Diseases Source Type: research
Conclusions: The study concluded in bringing out the contributing risk factors for brucellosis. The study concluded that treating animal infection on their own and helping animals during reproduction without using protective gear, as major contributing risk for brucellosis. Other factors includes, keeping animals in close proximity during sleep, irregular vaccination, etc.
Source: Indian Journal of Community Medicine - Category: International Medicine & Public Health Authors: Source Type: research
In this study, the gene encoding OMP19 antigen was primarily amplified and cloned into an expression vector called pT1NX, and then transformed to L. casei cell via electroporation technique. The expression was confirmed using specific antibody against the recombinant protein via immunological screening tests such as western blot and immunofluorescence assay. Finally, recombinant L. casei was orally fed to mice and the results were further recorded, indicating that the mice group which received OMP19 through L. casei based vaccine represented a very good general and mucosal immune responses protective against challenges wit...
Source: Comparative immunology, microbiology and infectious diseases. - Category: Allergy & Immunology Authors: Tags: Comp Immunol Microbiol Infect Dis Source Type: research
More News: Biochemistry | Brucellosis