Rapid and combined detection of Mycoplasma pneumoniae, Epstein-Barr virus and human cytomegalovirus using AllGlo quadruplex quantitative PCR.

Rapid and combined detection of Mycoplasma pneumoniae, Epstein-Barr virus and human cytomegalovirus using AllGlo quadruplex quantitative PCR. J Med Microbiol. 2016 Apr 19; Authors: Chen Y, He H, Pan P, He S, Dong X, Chen Y, Wang S, Yu D Abstract Acute respiratory infections (ARI) cause substantial morbidity and mortality worldwide. The causes of ARI are dynamic, and co-infections of Mycoplasma pneumoniae (MP), Epstein-Barr virus (EBV) or human cytomegalovirus (HCMV) are recently developing ARI causes. Here, we established a quadruplex quantitative PCR (qPCR) method to rapidly identify and simultaneously detect a single infection or co-infection of these three pathogens and an internal control in a single tube using AllGlo probes. The analysis demonstrated a wide linear range of detection from 101 to 108 copies/test and a low coefficient of variation of less than 5%. The amplification efficiencies were all close to 1, and the correlation coefficients (r2) were all greater than 0.99. We found no significant difference in a comparative reagent test (P > 0.05). Moreover, the results of tests on clinical samples using AllGlo quadruplex qPCR and TaqMan uniplex qPCR were in near perfect agreement (kappa value = 0.97). Clinically, the availability of this method will enable better differential diagnosis, disease surveillance and controlled outcomes. PMID: 27093597 [PubMed - as supplied by publisher]
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research