In vitro Evaluation of Copaifera oblongifolia Oleoresin against Bacteria Causing Oral Infections and assessment of its Cytotoxic Potential.

This study also investigated the bactericidal kinetics (time-kill curves) and the synergistic effect of the C. oblongifolia oleoresin. Additionally, this study evaluated the cytotoxic activity of the oleoresin toward V79 cells by means of the colony-forming assay. The C. oblongifolia oleoresin gave promising MIC and MBC values, which ranged from 25 to 200 μg/mL. Analysis of the MBIC50 values of the oleoresin revealed it displayed biofilm inhibitory activity against all the assayed bacteria. Analysis of the bactericidal kinetics showed different behaviors of the oleoresin against the tested bacteria at the different time intervals and concentrations assayed in this study. An additive effect of the oleoresin with chlorhexidine dihydrochloride occurred only for S. mitis and A. actinomycetemcomitans. The C. oblongifolia oleoresin showed cytotoxic activity at concentrations ≥ 625 µg/mL.
PMID: 27087492 [PubMed - as supplied by publisher]

Source: Current Pharmaceutical Biotechnology - April 14, 2016 Category: Biotechnology Authors: da Silva Moraes T, Leandro LF, de Oliveira Silva L, Santiago MB, Souza AB, Furtado RA, Tavares DC, Veneziani RC, Ambrósio SR, Bastos JK, Martins CH Tags: Curr Pharm Biotechnol Source Type: research