Detection of pituitary antibodies by immunofluorescence. approach and results in patients with pituitary diseases.

DETECTION OF PITUITARY ANTIBODIES BY IMMUNOFLUORESCENCE. APPROACH AND RESULTS IN PATIENTS WITH PITUITARY DISEASES. J Clin Endocrinol Metab. 2014 Feb 25;:jc20141049 Authors: Ricciuti A, De Remigis A, Landek-Salgado MA, De Vincentiis L, Guaraldi F, Lupi I, Iwama S, Wand GS, Salvatori R, Caturegli P Abstract Context: Pituitary antibodies have been measured mainly to identify patients whose disease is caused or sustained by pituitary-specific autoimmunity. Although reported in over one hundred publications, they have yielded variable results and are thus considered of limited clinical utility. Objectives: To analyze all publications reporting pituitary antibodies by immunofluorescence for detecting the major sources of variability; to experimentally test these sources and devise an optimized immunofluorescence protocol; to assess prevalence and significance of pituitary antibodies in patients with pituitary diseases. Study design and outcome measures: We first evaluated the effect of pituitary gland species, section fixation, autofluorescence quenching, blockade of unwanted antibody binding, and use of purified immunoglobulins G on the performance of this antibody assay. We then measured cross-sectionally the prevalence of pituitary antibodies in 390 pituitary cases and 60 healthy controls, expressing results as present or absent and according to the (granular, diffuse, perinuclear, or mixed) staining pattern. Results: Human pituitary was the best substrate to de...
Source: The Journal of Clinical Endocrinology and Metabolism - Category: Endocrinology Authors: Tags: J Clin Endocrinol Metab Source Type: research

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