Detection of pituitary antibodies by immunofluorescence. approach and results in patients with pituitary diseases.
DETECTION OF PITUITARY ANTIBODIES BY IMMUNOFLUORESCENCE. APPROACH AND RESULTS IN PATIENTS WITH PITUITARY DISEASES. J Clin Endocrinol Metab. 2014 Feb 25;:jc20141049 Authors: Ricciuti A, De Remigis A, Landek-Salgado MA, De Vincentiis L, Guaraldi F, Lupi I, Iwama S, Wand GS, Salvatori R, Caturegli P Abstract Context: Pituitary antibodies have been measured mainly to identify patients whose disease is caused or sustained by pituitary-specific autoimmunity. Although reported in over one hundred publications, they have yielded variable results and are thus considered of limited clinical utility. Objectives: To analyze all publications reporting pituitary antibodies by immunofluorescence for detecting the major sources of variability; to experimentally test these sources and devise an optimized immunofluorescence protocol; to assess prevalence and significance of pituitary antibodies in patients with pituitary diseases. Study design and outcome measures: We first evaluated the effect of pituitary gland species, section fixation, autofluorescence quenching, blockade of unwanted antibody binding, and use of purified immunoglobulins G on the performance of this antibody assay. We then measured cross-sectionally the prevalence of pituitary antibodies in 390 pituitary cases and 60 healthy controls, expressing results as present or absent and according to the (granular, diffuse, perinuclear, or mixed) staining pattern. Results: Human pituitary was the best substrate to de...
Conclusions: Our report is the first study to examine the distant metastatic breast cancer proteome using FFPE tissues. The depth of our dataset allowed us to discover a novel biomarker candidate and a proteomic characteristics of distant metastatic breast cancer. Distinct molecular features of various breast cancer subtypes were also established. Our proteomic data constitute a valuable resource for research on distant metastatic breast cancer. PMID: 32489334 [PubMed]
Publication date: September 2020Source: Research in Developmental Disabilities, Volume 104Author(s): Jennifer M. Glennon, Hana D’Souza, Luke Mason, Annette Karmiloff-Smith, Michael S.C. Thomas
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This article summarizes some clinical shortcuts. Simple procedures such as taking a detailed medical history can provide clues to the underlying cause of chronic pruritus in order to achieve targeted diagnostic workup or to avoid unnecessary testing. If clinical shortcuts are not identified, we recommend a structured medical history, which is also discussed in this article. PMID: 32488462 [PubMed - as supplied by publisher]
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