A Low-Cost Method for Tracking the Induction of Apoptosis Using FRET-Based Activity Sensors in Suspension Cells

Apoptosis, or programmed cell death, is a tightly regulated cellular event that plays an important role in both normal developmental processes and many pathological states. The induction of apoptosis is tightly regulated through the coordinated action of members of the caspase family of proteases. Here we discuss a relatively inexpensive protocol for monitoring the induction and progression of apoptosis using a genetically encoded fluorescence resonance energy transfer (FRET)-based biosensor of the executioner caspase, caspase-3, in living suspension cells.
Source: Springer protocols feed by Pharmacology/Toxicology - Category: Drugs & Pharmacology Source Type: news