Red-fluorescent Activatable Probes for the Detection of Hydrogen Peroxide in Living Cells.
Red-fluorescent Activatable Probes for the Detection of Hydrogen Peroxide in Living Cells. Comb Chem High Throughput Screen. 2016 Apr 8; Authors: Garcia-Guzman C, Fernandez A, Avlonitis N, Bradley M, Vendrell M Abstract Many inflammatory processes are associated with an increase in the production of reactive oxygen species (ROS). Chemical probes that specifically detect ROS are potentially useful tools for the early diagnosis of inflammatory diseases as well as cancer. Herein we have developed a library of coumarin hybrids by condensation of various heterocyclic quaternary salts to a 7-hydroxycoumarin scaffold. From our library we identified one benzothiazole-coumarin hybrid as a red-fluorescent compound with emission maxima around 620 nm and a strong fluorogenic response. Furthermore, we proved that this scaffold is suitable for the preparation of activatable probes, such as by modification with a boronate group for selective sensing of hydrogen peroxide (H2O2). In vitro assays confirmed the reactivity and subsequent emission of our probe upon incubation with H2O2 with good selectivity over different ROS and reactive nitrogen species (RNS) as well as minimal toxicity in cells. Finally cell imaging experiments were performed in murine macrophages and validated the utility of the activatable probe for the detection of H2O2 in living cells. PMID: 27055749 [PubMed - as supplied by publisher]
Publication date: Available online 4 December 2019Source: The Lancet Global HealthAuthor(s): Marc Arbyn, Elisabete Weiderpass, Laia Bruni, Silvia de Sanjosé, Mona Saraiya, Jacques Ferlay, Freddie BraySummaryBackgroundThe knowledge that persistent human papillomavirus (HPV) infection is the main cause of cervical cancer has resulted in the development of prophylactic vaccines to prevent HPV infection and HPV assays that detect nucleic acids of the virus. WHO has launched a Global Initiative to scale up preventive, screening, and treatment interventions to eliminate cervical cancer as a public health problem during th...
Publication date: Available online 4 December 2019Source: The Lancet Global HealthAuthor(s): Afsan Bhadelia
CONCLUSIONS: Both low and high Hgb levels were associated with an increased risk of death from various causes, and some diseases showed different patterns according to sex. PMID: 31795616 [PubMed - in process]
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Contributor : Janet L StanfordSeries Type : Expression profiling by arrayOrganism : Homo sapiensPrognostic biomarkers are useful to screen patients with clinically localized prostate cancer (PCa) who are at high risk of metastatic progression. The tumor transcriptome can be used to evaluate the aggressiveness of PCa and predict adverse patient outcomes. Genomewide gene expression levels were measured in primary tumor samples of 503 patients in a population ‐based cohort.
This study demonstrated that a homogeneous polysaccharide (HPS) extracted from Hawthorn exerted anti-cancer effects on colon cancer cells. Human colon cancer cell line HCT116 were treated with 125–1000 µg/mL HPS for 12 h and subsequent analysis was performed on proliferation and signaling pathways. HPS significantly inhibited proliferation of HCT116 cells when analyzed using WST-1 assay and immunofluorescence staining of Ki-67. Flow cytometric studies revealed that HPS could arrest the cell cycle in the S and G2/M phases and increase the rate of apoptosis. HPS downregulated the expression of Cyclin A1...
This study assessed dark sweet cherry (DSC) phenolics as chemopreventive/chemotherapeutics against aggressive breast cancer subtypes.DSC phenolics in whole extract (WE) and fractions enriched in phenolic acids (F1), flavonols (F3) and proanthocyanidins (F4) inhibited breast cancer cell growth with potency MDA-MB-453 > MDA-MB-231 ∼ BT-474, without toxicity to MCF-10A normal breast cells, except F3 that inhibited BT-474 and MCF-10A cells with similar toxicity. DSC anthocyanins (F2) inhibited breast cancer cell lines with similar potencies without toxicity to MCF-10A cells.Mechanisms for WE, F2 and F4 breast an...