Expression of prostaglandin receptors in Chlamydia trachomatis-infected recurrent spontaneous aborters.

Expression of prostaglandin receptors in Chlamydia trachomatis-infected recurrent spontaneous aborters. J Med Microbiol. 2016 Mar 30; Authors: Singh N, Prasad P, Singh LC, Das B, Rastogi S Abstract Study was undertaken to quantify the expression of Prostaglandin (PG) receptors and find the effect of gestational age on expression of PG receptor genes in Chlamydia trachomatis-infected Recurrent Spontaneous Aborters (RSA). Endometrial Curettage Tissue (ECT) was collected from 130 RSA (Group-I) and 100 age-matched controls (Group-II) at Department of Obstetrics and Gynecology, SJH, New Delhi (India). PCR was performed for diagnosis of C. trachomatis cryptic plasmid; mRNA expression of PG receptor genes was assessed by real-time PCR (q-PCR), while serum progesterone/ estrogen were determined by respective commercial kits. Data was statistically evaluated. 15.4% RSA (Group-I) were diagnosed as C. trachomatis-positive (200 bp), whereas controls were uninfected. q-PCR showed significant upregulation ('p' < 0.0001) of PGE-2 (EP-1, EP-2, EP-3, EP-4), PGF2α (FP) and PGI2 (IP) receptors in Group-I versus Group-II. The expression of PG receptors increased significantly with advanced gestational age (**'p' < 0.002), however, only contractile receptors, EP-1, EP-3 and FP were positively correlated with gestational age in Group-I. In infected-RSA, mean serum progesterone level was significantly low (***'p' < 0.0001) while serum estrogen wa...
Source: Journal of Medical Microbiology - Category: Microbiology Authors: Tags: J Med Microbiol Source Type: research