Mitochondria are required for pro-ageing features of the senescent phenotype

Cell senescence is an important tumour suppressor mechanism and driver of ageing. Both functions are dependent on the development of the senescent phenotype, which involves an overproduction of pro-inflammatory and pro-oxidant signals. However, the exact mechanisms regulating these phenotypes remain poorly understood. Here, we show the critical role of mitochondria in cellular senescence. In multiple models of senescence, absence of mitochondria reduced a spectrum of senescence effectors and phenotypes while preserving ATP production via enhanced glycolysis. Global transcriptomic analysis by RNA sequencing revealed that a vast number of senescent-associated changes are dependent on mitochondria, particularly the pro-inflammatory phenotype. Mechanistically, we show that the ATM, Akt and mTORC1 phosphorylation cascade integrates signals from the DNA damage response (DDR) towards PGC-1β-dependent mitochondrial biogenesis, contributing to a ROS-mediated activation of the DDR and cell cycle arrest. Finally, we demonstrate that the reduction in mitochondrial content in vivo, by either mTORC1 inhibition or PGC-1β deletion, prevents senescence in the ageing mouse liver. Our results suggest that mitochondria are a candidate target for interventions to reduce the deleterious impact of senescence in ageing tissues.
Source: EMBO Journal - Category: Molecular Biology Authors: Tags: Ageing, Cell Cycle, Metabolism Articles Source Type: research

Related Links:

Conclusions: The patients demonstrated a high level of safety, efficacy, and satisfaction with the ileal-vaginal extension for treating vaginal stump contracture after cervical cancer surgery combined with radiotherapy, suggesting that this method is valid for broad clinical application to improve the life quality of cervical cancer patients after surgery. PMID: 31739704 [PubMed - as supplied by publisher]
Source: Journal of Investigative Surgery - Category: Surgery Tags: J Invest Surg Source Type: research
Publication date: Available online 19 November 2019Source: Protein Expression and PurificationAuthor(s): Chandler B. Est, Regina M. MurphyAbstractRetinol binding protein IV (RBP) functions as the principal carrier of retinol (Vitamin A) in the blood, where RBP circulates bound to another serum protein, transthyretin. Isolation of pure RBP from the transthyretin complex in human serum can be difficult, but expression of RBP in recombinant systems can circumvent these purification issues. Human recombinant RBP has previously been successfully expressed and purified from E. coli, but recovery of active protein typically requi...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
In this study, we demonstrate that a His-tag impacts DNA complex formation by the C-terminal domain of the ??-subunit (??CTD) of Helicobacter pylori RNA polymerase in a metal-dependent fashion. The ??CTD was purified with a cleavable His-tag, and complex formation between αCTD, the nickel-responsive metalloregulator HpNikR, and DNA was investigated using electrophoretic mobility shift assays. An interaction between His-tagged ??CTD (His??CTD) and the HpNikR-DNA complex was observed; however, this interaction was not observed upon removal of the His-tag. Further analysis revealed that complex formation between His&alp...
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research
This study aimed to compare the Vitamin D status and serum 25(OH)D concentrations of adults living in an urban area to adults living in a rural area in the West of Ireland (latitude 53.27 °North). A cross-sectional retrospective analysis of clinical records was performed. Following interrogation of the electronic laboratory information system, individuals who had Vitamin D samples analysed at Galway University Hospitals between January 2011 and December 2015 were identified. Clinical demographics, setting and date of sampling were recorded. In total, 17,590 patients (urban n = 4,824; rural n&thinsp...
Source: The Journal of Steroid Biochemistry and Molecular Biology - Category: Biochemistry Source Type: research
In this study, roles of GhFSN5 were dissected. qRT-PCR analysis showed that GhFSN5 was predominantly transcribed during the fiber SCW thickening stage. In addition, a large number of fiber SCW biosynthetic genes and SCW-related TFs were co-expressed with GhFSN5. Heterologous expression of GhFSN5 in Arabidopsis resulted in plants with smaller siliques and severe sterility. Anther dehiscence in transgenic lines was not substantially affected, but most pollen was collapsed and nonviable. Furthermore, cellulose and lignin contents in inflorescence stems as well as roots were reduced in transgenic lines, compared with the wild ...
Source: Plant Physiology and Biochemistry - Category: Biochemistry Source Type: research
Publication date: Available online 19 November 2019Source: Plant Physiology and BiochemistryAuthor(s): Sundus Zafar, Min-Qiang Tang, Yu-Kang Wang, Rehman Sarwar, Sheng-Yi Liu, Xiao-Li TanAbstractBrassica napus (rapeseed) serves as a main source of edible oil, and the oil's quality is mainly determined by the relative proportions of fatty acids. A high oleic acid concentration in B. napus oil increases its shelf life and oxidative stability. Therefore, attaining a high oleic acid concentration is necessary to enhance the nutritional quality of rapeseed oil. Here, an association study of candidate genes was conducted using a...
Source: Plant Physiology and Biochemistry - Category: Biochemistry Source Type: research
In this study, the promoter of iturin A synthetase cluster of Bacillus amyloliquefaciens HZ-12 was respectively replaced by promoters P43, PbacA, PsrfA and Pylb, our results implied that transcriptional level of gene ituD and iturin A titer showed consistent change trends, and PbacA was proven as the most efficient promoter, iturin A titer of which reached 950.08 ± 19.43 mg/L. Furthermore, regulator gene abrB was deleted to release the repression effect of AbrB on PbacA, ituD transcriptional level and iturin A titer were increased by 133.25% and 20.88%, respectively. Then, the maximum iturin A t...
Source: Process Biochemistry - Category: Biochemistry Source Type: research
Publication date: Available online 19 November 2019Source: Process BiochemistryAuthor(s): Ping Ying Lye, Suriani Mohd Noor, Syamsiah Aini Shohaimi, Niny Fariza Junoh, Soo Choon Tan, Shinichi Iwamoto, Eiji Kotani, Mohd Nor Norazmi, Toshihiro Nagamine, Hajime Mori, Mervyn W.O. LiewABSTRACTRecombinant hemagglutinin-neuraminidase (HN) based subunit vaccine, which is non-infectious and can be produced using insect cell-culture systems, is a potential alternative to conventional live and inactivated Newcastle disease virus (NDV) vaccines. However, process development for manufacture and efficacy assessment of HN subunit vaccines...
Source: Process Biochemistry - Category: Biochemistry Source Type: research
In this study, we are successfully fabricated on a hydrogel consisting of TiO2 nanoparticles loaded onto a gelatin/chitosan matrix to control the acceleration of bone fracture healing and improved the nursing care applications. Each specimen (chitosan, gelatin and titanium dioxide) were characterized and confirmed by using different techniques, Fourier transforms infrared spectroscopy, X-ray diffraction analysis, Scanning Electron Microscopy with Elemental dispersive X-ray analysis, Thermo-gravimetric and Differential thermal analysis. In addition, the cell cytotoxicity results verified that the TiO2/gelatin-chitosan hydro...
Source: Process Biochemistry - Category: Biochemistry Source Type: research
In this study, susceptibility to nine insecticides was compared among orange/yellow, gray/brown and blue/green morphs of adult D. citri. Imidacloprid and lambda-cyhalothrin resulted in higher mortality in the orange/yellow morph than in the blue/green morph. The orange/yellow morph was significantly more susceptible to acetamiprid, bifenthrin and chlorfenapyr than the blue/green and gray/brown morphs. For clothianidin and chlorpyriphos, the orange/yellow morph was significantly more susceptible than the gray/brown morphs. Susceptibility to dinotefuran and thiamethoxam was not significantly different between the three color...
Source: Pesticide Biochemistry and Physiology - Category: Biochemistry Source Type: research
More News: Liver | Mitochondria | Molecular Biology | Urology & Nephrology